Sanz-Martin, I.Doolittle-Hall, J.Teles, R. P.Pate, M.Belibasakis, G. N.Hämmerle, C. H.Jung, R. E.Teles, F. R.F.2023-05-222023-05-222017-12-012021-06-01https://repository.upenn.edu/handle/20.500.14332/9400Aim To compare the microbiome of healthy (H) and diseased (P) peri-implant sites and determine the core peri-implant microbiome. Materials and Methods Submucosal biofilms from 32 H and 35 P sites were analyzed using 16S rRNA sequencing (MiSeq, Illumina), QIIME and HOMINGS. Differences between groups were determined using Principal Coordinate Analysis (PCoA), t-tests and Wilcoxon rank sum test and FDR-adjusted. The peri-implant core microbiome was determined. Results PCoA showed partitioning between H and P at all taxonomic levels. Bacteroidetes, Spirochetes and Synergistetes were higher in P, while Actinobacteria prevailed in H (p<0.05). Porphyromonas and Treponema were more abundant in P and while Rothia and Neisseria were higher in H (p<0.05). The core peri-implant microbiome contained Fusobacterium, Parvimonas and Campylobacter sp. T. denticola and P. gingivalis levels were higher in P, as well as F. alocis, F fastidiosum and T. maltophilum (p<0.05). Conclusion The peri-implantitis microbiome is commensal-depleted and pathogen-enriched, harboring traditional and new pathogens. The core peri-implant microbiome harbors taxa from genera often associated with periodontal inflammation.This is the peer reviewed version of the following article: [Sanz‐Martin, I., Doolittle‐Hall, J., Teles, R. P., Patel, M., Belibasakis, G. N., Hämmerle, C. H. F., . . . Teles, F. R. F. (2017). Exploring the microbiome of healthy and diseased peri‐implant sites using illumina sequencing. Journal of Clinical Periodontology, 44(12), 1274-1284. doi:10.1111/jcpe.12788]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.microbiomedental implantperi-implantitisDNAperiodontalsequencingDentistryArticle