Heller, Elizabeth AGraves, Laurel APack, Allan IAbel, Ted2023-05-232023-05-232003-01-012018-01-22https://repository.upenn.edu/handle/20.500.14332/42606Many behavioral and electrophysical studies in animals and humans have suggested that sleep and circadian rhythms influence memory consolidation. In rodents, hippocampus-dependent memory may be particularly sensitive to sleep deprivation after training, as spatial memory in the Morris water maze is impaired by rapid eye movement sleep deprivation following training. Spatial learning in the Morris water maze, however, requires multiple training trials and performance, as measured by time to reach the hidden platform is influenced by not only spatial learning but also procedural learning. To determine if sleep is important for the consolidation of a single-trial, hippocampus-dependent task, we sleep deprived animals for 0-5 and 5-10 h after training for contextual and cued fear conditioning. We found that sleep deprivation from 0-5 h after training for this task impaired memory consolidation for contextual fear conditioning whereas sleep deprivation from 5-10 h after training had no effect. Sleep deprivation at either time point had no effect on cued fear conditioning, a hippocampus-independent task. Previous studies have determined that memory consolidation for fear conditioning is impaired when protein kinase A and protein synthesis inhibitors are administered at the same time as when sleep deprivation is effective, suggesting that sleep deprivation may act by modifying these molecular mechanisms of memory storage.Originally published in Learning and Memory © 2003 Cold Spring Harbor Laboratory Press. Now available Open Access under a Creative Commons Attribution-NonCommercial 4.0 License (CC BY-NC 4.0).Animal Experimentation and ResearchBiochemistry, Biophysics, and Structural BiologyNeuroscience and NeurobiologyPharmacy and Pharmaceutical SciencesSleep MedicineTranslational Medical ResearchReport